EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit from EpiGentek

Product Specs
ItemEpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit
CompanyEpiGentek
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP-1038
Quantity24 Reactions, 48 Reactions, 96 Reactions
ApplicationsImmunoprecipitation
FormatMicroplate-based
Sample TypeDNA
Research AreaDNA Methylation
SourceCell/Tissue
Nucleic Acid TypeMethylated DNA, ChIP DNA

EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

United States

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(1) Tet2 regulates Sin3a recruitment at active enhancers in embryonic stem cellsiScienceJuly 21, 2023Flores JC, Sidoli S, Dawlaty MM.strand,Invitrogen,18080-400 XtremeGene 9 DNA transfection reagent,Roche,06365787001 EpiQuik hMeDIP kit,Epigentek,P-1038-24 ,, Deposited data,Deposited data,Deposited data ,, RNA-seq,This paper,GEO:GSE213398 CUT&Tag,This

(2) RASAL2 Deficiency Attenuates Hepatic Steatosis by Promoting Hepatic VLDL Secretion via the AKT/TET1/MTTP AxisJournal of Clinical and Translational HepatologyApril 28, 2023Hao Ding, Jiang-Hong Yu, Ge Ge, Yan-Yun Ma, Jiu-Cun Wang, Jun Zhang, Jie LiuDNA Immunoprecipitation (hMeDIP) 5-hmC immunoprecipitation was performed with EpiQuik hmeDIP kits (P-1038; Epigentek, Farmingdale, NY, USA). Genomic DNA was extracted and fragmented by sonication. DNA containing

(3) The Polyunsaturated Fatty Acid EPA, but Not DHA, Enhances Neurotrophic Factor Expression through Epigenetic Mechanisms and Protects against Parkinsonian Neuronal Cell DeathInternational Journal of Molecular SciencesDecember 19, 2022Maria Rachele Ceccarini, Veronica Ceccarelli, Michela Codini, Katia Fettucciari, Mario Calvitti, Samuela Cataldi, Elisabetta Albi, Alba Vecchini, Tommaso Beccari200–1000 bp in size) was denatured for 10 min at 95 °C. Hydroxymethylated DNA immunoprecipitation (hMeDIP; P-1038-24; Epigentek) and methylated DNA immunoprecipitation (MeDIP; P-2019-24; Epigentek) assays were performed

(4) Deficiency of Tet3 in nucleus accumbens enhances fear generalization and anxietyâ€like behaviors in miceBrain PathologyNovember 1, 2022Buâ€Fang Fan, Bo Hao, Yunâ€Da Dai, Li Xue, Yanâ€Wei Shi, Lu Liu, Shouâ€Min Xuan, Ning Yang, Xiaoâ€Guang Wang, Hu Zhaoand 5mC were assessed by EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (Epigentek, P‐1038) and MethylFlash Methylated DNA Quantification Kit (Epigentek, P‐1034) after DNA extraction from mice

(5) Regulation of Rac1 transcription by histone and DNA methylation in diabetic retinopathyScientific ReportsDecember 1, 2021Renu A. Kowluru, Rakesh Radhakrishnan, Ghulam Mohammadlevel of 5hmC was quantified using hydroxymethylated DNA Immunoprecipitation (hMeDIP) kit (Cat. No. P-1038, EPIGENTEK, Farmingdale, NY, USA). The enrichment of 5hmC at the Rac1 promoter was analyzed by qRT-PCR

(6) Retinopathy in a Diet-Induced Type 2 Diabetic Rat Model and Role of Epigenetic ModificationsDiabetesApril 1, 2020Renu A. Kowluruusing EpiQuik methylated and hydroxymethylated DNA Immunoprecipitation Kits (catalog numbers P-1015 and P-1038, respectively, from EpiGentek, Farmingdale, NY), as described previously (19,24). The binding of Tet2,

(7) Homocysteine Disrupts Balance between MMP-9 and Its Tissue Inhibitor in Diabetic Retinopathy: The Role of DNA MethylationInternational Journal of Molecular SciencesMarch 5, 2020Ghulam Mohammad, Renu A. Kowlurugenomic DNA by MethyLamp Methylated and Hydroxymethylated DNA capture Kits (Cat. No. P-1015 and Cat. No. P-1038 respectively, EPIGENTEK), as reported previously [45,46], using the primers targeting MMP-9 and Timp1

(8) Hepatic TET3 contributes to type-2 diabetes by inducing the HNF4α fetal isoformNature communicationsJanuary 17, 2020Da Li, Cao T, Sun X, Jin S, Di Xie, Huang X, Yang X, Carmichael GG, Taylor HS, Diano S, et al.immunoprecipitation coupled with qPCR The experiments were carried out using the EpiQuik hMeDIP Kit (P-1038-48, Epigentek) according to the manufacturer’s instructions. Briefly, mouse primary hepatocytes maintained

(9) Epigenetic Modifications in Peripheral Blood as Potential Noninvasive Biomarker of Diabetic RetinopathyTranslational Vision Science & TechnologyDecember 18, 2019Arul J. Duraisamy, Rakesh Radhakrishnan, Berhane Seyoum, Gary W. Abrams, Renu A. Kowluruhydroxymethylated DNA immunoprecipitation kits (MeDIP and hMeDIP kits respectively, Cat. No. P-1015, P-1038; EpiGentek, Farmingdale, NY). Using 250 ng gDNA for 5mC and 500 ng for 5hmC, DNA bound to the high DNA

(10) EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoterTheranosticsJanuary 1, 2019Shi F, Zhou M, Shang L, Du Q, Li Y, Xie L, Liu X, Tang M, Luo X, Fan J, et al.hMeDIP assay was performed using the EpiQuik™ Hydroxymethylated DNA immunoprecipitation assay kit (Cat: P1038, EpigenTek). Fumarate, α-KG, succinate, and 2-HG assays α-KG and 2-HG were measured using the alpha-ketoglutarate

(11) Epigenetics and Regulation of Oxidative Stress in Diabetic RetinopathyInvestigative Opthalmology & Visual ScienceOctober 23, 2018Arul J. Duraisamy, Manish Mishra, Anjaneyulu Kowluru, Renu A. Kowluruhydroxymethyldated DNA Immunoprecipitation (MeDIP)/(hMeDIP) kits, respectively (Cat. Nos. P-1015 and P-1038, respectively; EPIGENTEK, Farmingdale, NY, USA). The enrichment of 5mC or 5hmC at the Rac1 promoter

(12) Selective inhibition of CTCF binding by iAs directs TET-mediated reprogramming of 5-hydroxymethylation patterns in iAs-transformed cellsToxicology and applied pharmacologyJanuary 1, 2018Rea M, Gripshover T, Fondufe-Mittendorf Y.Blood and Tissue Kit (Qiagen 69506) and 5hme-DIP was performed using the EpiQuik hMeDIP Kit (Epigentek P-1038) following the manufacturer’s protocol. 2 µL of eluate was used in qRT-PCR reactions to determine changes

(13) TREM2 upregulation correlates with 5-hydroxymethycytosine enrichment in Alzheimer’s disease hippocampusClinical EpigeneticsDecember 1, 2016Naiara Celarain, Javier Sánchez-Ruiz de Gordoa, María Victoria Zelaya, Miren Roldán, Rosa Larumbe, Laura Pulido, Carmen Echavarri, Maite Mendioroz

(14) Dynamic DNA methylation of matrix metalloproteinase-9 in the development of diabetic retinopathyLaboratory InvestigationOctober 1, 2016Renu A. Kowluru, Yang Shan, Manish Mishra

(15) The control region of mitochondrial DNA shows an unusual CpG and non-CpG methylation patternDNA research : an international journal for rapid publication of reports on genes and genomesDecember 1, 2013Dina Bellizzi, Patrizia D'Aquila, Teresa Scafone, Marco Giordano, Vincenzo Riso, Andrea Riccio, Giuseppe Passarino

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